In recent years, manganese (Mn) offers great advantages as a T 1-weighted MR contrast agent attributed to the presence of five unpaired electron in the bivalent state. Therefore, the combination of MRI and FL imaging renders a promising dual-modal imaging technique with complementary characteristics to improve the accuracy of tumor diagnosis. Fluorescence imaging, as a functional imaging method, could compensate the limitation of MR imaging for its high sensitivity in cellular and subcellular levels. Among these imaging methods, MR imaging is a powerful technique for high spatially resolved visualization of the anatomic structure and function of soft tissues with a deep penetration, but it has the disadvantage of low sensitivity. Till date, various imaging techniques have been proposed, including magnetic resonance imaging (MRI), fluorescence imaging (FL), computed tomography imaging (CT), position emission tomography (PET) and photoacoustic imaging (PA), etc. It could integrate the merits of different imaging techniques to improve the effectiveness and sensitivity of the diagnosis. Recently, multimodal imaging based on molecular nanoprobe has gained increasing interests for the specific and accurate diagnosis of the tumor. Therefore, developing a imaging probe to realize early and accurate diagnosis of the ovarian carcinoma is urgently needed for improving the therapeutic efficacy and the lifespan of the patients. However, most of ovarian carcinomas have progressed to the advanced stages (FIGO III ~ IV) even with a distant metastasis upon their diagnosis.
The 5-year survival rate was reported to be 92% for the early ovarian carcinoma patients with effective treatments. The prepared with excellent biocompatibility, high-performance and superior tumor-targeting ability provides a novel fluorescence/MR dual-modal nanoprobe for specific labeling and detection of ovarian carcinoma cells in vitro and in vivo.Īs reported, ovarian carcinoma is a common and deadly gynecologic cancer with an incidence of approximately 25 ~ 30% in the female genital cancers. Besides, the high biocompatibility and easy excretion from the body of the nanoprobe were further confirmed in vivo. Benefiting from the high r 1 relaxivity, the nanoprobe exhibited targeted and enhanced MR contrast effect in the ovarian carcinoma cells and tumor bearing mice model.
Encouraged by the tunable photoluminiscence of the nanoprobe and Anti-HE4 targeting ligand, the ovarian carcinoma cells were specifically labeled by the nanoprobe with multi-color fluorescences. The prepared Mn-N-CNSs revealed excellent aqueous dispersity, good colloidal stability, great optical properties and high longtudinal relaxivity rate (r 1 = 10.30 mM −1 s −1). In this study, manganese-nitrogen doped carbon nanosheets (Mn-N-CNSs) coupled with Anti-HE4 monoclonal antibody were synthesized for the specific and targeted fluorescence/MR dual-modal imaging of ovarian carcinoma. Development of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving the therapeutic efficacy and the lifespan of the patients.
0 kommentar(er)
